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In western societies, colorectal cancer is one of the main causes of cancer-related deaths among adults. Every year, around 130,000 patients in the European Union are confronted with the diagnosis of this disease. Because the cure rates are high for local tumors (90% for Dukes A and 60-80% for Dukes B) but low for advanced or metastasized disease tumors (30-60% for Dukes C and 5% for Dukes D), great effort must be undertaken to detect early stages of colorectal cancer and its precursors, colorectal adenomas.

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Designation Medium Method Tests Art. Nr.
Hemoglobin ELISA Stool ELISA 96 01-10.010

Two polyclonal antibodies are used in the hemoglobin ELISA that are highly specific against human hemoglobin.  A microtiter plate is coated with a polyclonal rabbit antibody geared against hemoglobin.  Standards, controls, and samples to be determined are pipetted in the "wells."  Following an incubation and a wash phase, a polyclonal antibody marked with HRP (Horseradish-Peroxidase) is added.  After a further incubation and wash phase, the substrate (Tetramethylbenzidin) for the enzyme reaction is added.  The color reaction is halted through the addition of 0.5 M sulfuric acid (stop solution) and measured with a photometer with a wave length of 450 nm.  The concentration of hemoglobin is proportional to the intensity of the coloration. Using the standard curve, which is also measured, the concentration of the samples is calculated.

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Hemoglobin 1-point-calibration Stool ELISA 96 01-12.010

Two polyclonal antibodies are used in the hemoglobin ELISA that are highly specific against human hemoglobin.  A microtiter plate is coated with a polyclonal rabbit antibody geared against hemoglobin.  Standards, controls, and samples to be determined are pipetted in the "wells."  Following an incubation and a wash phase, a polyclonal antibody marked with HRP (Horseradish-Peroxidase) is added.  After a further incubation and wash phase, the substrate (Tetramethylbenzidin) for the enzyme reaction is added.  The color reaction is halted through the addition of 0.5 M sulfuric acid (stop solution) and measured with a photometer with a wave length of 450 nm.  The concentration of hemoglobin is proportional to the intensity of the coloration. Using the standard curve, which is also measured, the concentration of the samples is calculated.

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Hemoglobin EIA Stool EIA 96 01-11.010

One polyclonal antibody is used in the hemoglobin EIA that is highly specific against human hemoglobin . A polystyrol ball was coated with a polyclonal rabbit antibody geared against hemoglobin.  Standards, controls, and samples to be determined are pipetted in the tubes together with a ball and the antigene (hemoglobin) labled with HRP (Horseradish-Peroxidase). After a incubation and wash phase, the substrate (Tetramethylbenzidin) for the enzyme reaction is added.  The color reaction is halted through the addition of 0.5 M sulfuric acid (stop solution) and measured with a photometer with a wave length of 450 nm.  The concentration of hemoglobin is proportional to the intensity of the coloration. Using the standard curve, which is also measured, the concentration of the samples is calculated.
 

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Hemoglobin LIA Stool LIA 100 01-01.010

One polyclonal antibody is used in the hemoglobin LIA that is highly specific against human hemoglobin . A polystyrol ball was coated with a polyclonal rabbit antibody geared against hemoglobin.  Standards, controls, and samples to be determined are pipetted in the tubes together with a ball. The antigene (hemoglobin) labled with acridiniumester is added. An incubation and wash phase follows. Now the light emission is measured with a luminometer.  The concentration of hemoglobin is proportional to the height of light emission.  Using the standard curve, which was also measured, the concentration of the samples is calculated.

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Hemoglobin-Haptoglobin-Complex ELISA Stool ELISA 96 01-10.011

In hemoglobin-haptoglobin ELISA, a polyclonal antibody is used against haptoglobin and two monoclonal antibodies that are highly specific against human hemoglobin.  A microtiter plate is coated with a polyclonal rabbit antibody geared against haptoglobin. Standards, controls, and samples to be determined are pipetted in the "wells."  Following an incubation and a wash phase, a monoclonal antibody marked with HRP (Horseradish-Peroxidase) is added.  After a further incubation and wash phase, the substrate (Tetramethylbenzidin) for the enzyme reaction is added.  The color reaction is halted through the addition of 0.5 M sulfuric acid (stop solution) and measured with a photometer with a wave length of 450 nm.  The concentration of hemoglobin-haptoglobin is proportional to the intensity of the coloration. Using the standard curve, which is also measured, the concentration of the samples is calculated.

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Hemoglobin-Haptoglobin-Complex (1-point) Stool ELISA 96 01-12.011

In hemoglobin-haptoglobin ELISA, a polyclonal antibody is used against haptoglobin and two monoclonal antibodies that are highly specific against human hemoglobin.  A microtiter plate is coated with a polyclonal rabbit antibody geared against haptoglobin. Calibrator, controls, and samples to be determined are pipetted in the "wells."  Following an incubation and a wash phase, a monoclonal antibody marked with HRP (Horseradish-Peroxidase) is added.  After a further incubation and wash phase, the substrate (Tetramethylbenzidin) for the enzyme reaction is added.  The color reaction is halted through the addition of 0.5M sulfuric acid (stop solution) and measured with a photometer with a wave length of 450nm.  The concentration of hemoglobin-haptoglobin is proportional to the intensity of the coloration.  Using the calibrator, which is also measured, the concentration of the samples is calculated.

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Hemoglobin-Haptoglobin-Complex ILMA Stool ILMA 100 01-02.011

Two monoclonal that are specific against haptoglobin and one polyclonal antibody that is highly specific against human hemoglobin are used in the hemoglobin-haptoglobin-complex-ILMA. A polystyrol ball was coated with a polyclonal rabbit antibody geared against haptoglobin.  Standards, controls, and samples to be determined are pipetted in the tubes together with a coated ball.  Following incubation and wash phases, a monoclonal antibody marked with acridiniumester is added. Another incubation and wash step follows. Now the light emission is measured with a luminometer.  The concentration of the hemoglobin-haptoglobin-complex is proportional to the height of light emission.  Using the standard curve, which was also measured, the concentration of the samples is calculated.

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Haptoglobin ELISA Stool ELISA 96 01-10.012

Two polyclonal antibodies are used in the haptoglobin ELISA that are highly specific against human haptoglobin.  A microtiter plate is coated with a polyclonal rabbit antibody geared against haptoglobin. Standards, controls, and samples to be determined are pipetted in the "wells."  Following an incubation and a wash phase, a polyclonal antibody marked with HRP (Horseradish-Peroxidase) is added.  After a further incubation and wash phase, the substrate (Tetramethylbenzidin) for the enzyme reaction is added.  The color reaction is halted through the addition of 0.5 M sulfuric acid (stop solution) and measured with a photometer with a wave length of 450 nm.  The concentration of haptoglobin is proportional to the intensity of the coloration. Using the standard curve, which is also measured, the concentration of the samples is calculated.

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