» Assays
Food allergies and autoimmune diseases can be verified through an increased level of specific antibodies in stool. All immune globulin subclasses (IgA, IgG, IgE, and IgM) can be found there.
Designation | Medium | Method | Tests | Art. Nr. |
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Anti-Transglutaminase-ELISA | Stool | ELISA | 96 | 01-10.095 |
One polyclonal antibody is used in the anti-transglutaminase-ELISA. A microtiter plate is coated with transglutaminase. Standards, controls, and samples to be determined are pipetted in the "wells." Following an incubation and a wash phase, a polyclonal antibody marked with HRP (Horseradish-Peroxidase) is added. After a further incubation and wash phase, the substrate (Tetramethylbenzidin) for the enzyme reaction is added. The color reaction is halted through the addition of 0.5 M sulfuric acid (stop solution) and measured with a photometer with a wave length of 450 nm. The concentration of anti-transglutaminase is proportional to the intensity of the coloration. Using the standard curve, which is also measured, the concentration of the samples is calculated. |
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Anti-Transglutaminase (1-Punkt) | Stool | ELISA | 96 | 01-12.095 |
One polyclonal antibody is used in the anti-gliadin ELISA. A microtiter plate is coated with gliadin. Calibrator, controls, and samples to be determined are pipetted in the "wells." Following an incubation and a wash phase, a polyclonal antibody marked with HRP (Horseradish-Peroxidase) is added. After a further incubation and wash phase, the substrate (Tetramethylbenzidin) for the enzyme reaction is added. The color reaction is halted through the addition of 0.5 M sulfuric acid (stop solution) and measured with a photometer with a wave length of 450 nm. The concentration of anti-gliadin is proportional to the intensity of the coloration. Using the calibrator, which is also measured, the concentration of the samples is calculated. |
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Anti-Transglutaminase ILMA | Stool | ILMA | 100 | 01-02.095 |
One polyclonal antibody that is highly specific against human immunglobuline is used in the anti-transglutaminase-ILMA. A polystyrol ball was coated with transglutaminase. Standards, controls, and samples to be determined are pipetted in the tubes together with a coated ball. Following incubation and wash phases, a polyclonal antibody marked with acridiniumester is added. Another incubation and wash step follows. Now the light emission is measured with a luminometer. The concentration of anti-transglutaminse is proportional to the height of light emission. Using the standard curve, which was also measured, the concentration of the samples is calculated. |
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Anti-Gliadin ELISA | Stool | ELISA | 96 | 01-10.092 |
One polyclonal antibody is used in the anti-gliadin-ELISA. A microtiter plate is coated with gliadin. Standards, controls, and samples to be determined are pipetted in the "wells." Following an incubation and a wash phase, a polyclonal antibody marked with HRP (Horseradish-Peroxidase) is added. After a further incubation and wash phase, the substrate (Tetramethylbenzidin) for the enzyme reaction is added. The color reaction is halted through the addition of 0.5 M sulfuric acid (stop solution) and measured with a photometer with a wave length of 450 nm. The concentration of anti-gliadin is proportional to the intensity of the coloration. Using the standard curve, which is also measured, the concentration of the samples is calculated. |
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Anti-Gliadin (1-point-calibration) | Stool | ELISA | 96 | 01-12.092 |
One polyclonal antibody is used in the anti-gliadin-ELISA. A microtiter plate is coated with gliadin. Calibrator, controls, and samples to be determined are pipetted in the "wells." Following an incubation and a wash phase, a polyclonal antibody marked with HRP (Horseradish-Peroxidase) is added. After a further incubation and wash phase, the substrate (Tetramethylbenzidin) for the enzyme reaction is added. The color reaction is halted through the addition of 0.5 M sulfuric acid (stop solution) and measured with a photometer with a wave length of 450 nm. The concentration of anti-gliadin is proportional to the intensity of the coloration. Using the calibrator, which is also measured, the concentration of the samples is calculated. |
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Anti-Gliadin ILMA | Stool | ILMA | 100 | 01-02.092 |
One polyclonal antibody that is highly specific against human immunglobuline is used in the Anti-Gliadin-ILMA. A polystyrol ball is coated with gliadin. Standards, controls, and samples to be determined are pipetted in the tubes together with a coated ball. Following incubation and wash phases, a polyclonal antibody marked with acridiniumester is added. Another incubation and wash step follows. Now the light emission is measured with a luminometer. The concentration of anti-gliadin is proportional to the height of light emission. Using the standard curve, which is also measured, the concentration of the samples is calculated. |
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Anti-soy ELISA | Stool | ELISA | 96 | 01-10.096 |
One polyclonal antibody is used in the anti-soy ELISA. A microtiter plate is coated with soy. Standards, controls, and samples to be determined are pipetted in the "wells." Following an incubation and a wash phase, a polyclonal antibody marked with HRP (Horseradish-Peroxidase) is added. After a further incubation and wash phase, the substrate (Tetramethylbenzidin) for the enzyme reaction is added. The color reaction is halted through the addition of 0.5 M sulfuric acid (stop solution) and measured with a photometer with a wave length of 450 nm. The concentration of anti-soya is proportional to the intensity of the coloration. Using the standard curve, which is also measured, the concentration of the samples is calculated. |
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Anti-milk ELISA | Stool | ELISA | 96 | 01-10.097 |
One polyclonal antibody is used in the anti-milk ELISA. A microtiter plate is coated with milk. Standards, controls, and samples to be determined are pipetted in the "wells." Following an incubation and a wash phase, a polyclonal antibody marked with HRP (Horseradish-Peroxidase) is added. After a further incubation and wash phase, the substrate (Tetramethylbenzidin) for the enzyme reaction is added. The color reaction is halted through the addition of 0.5 M sulfuric acid (stop solution) and measured with a photometer with a wave length of 450 nm. The concentration of anti-milk is proportional to the intensity of the coloration. Using the standard curve, which is also measured, the concentration of the samples is calculated. |
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Anti-egg ELISA | Stool | ELISA | 96 | 01-10.098 |
One polyclonal antibody is used in the anti-egg ELISA. A microtiter plate is coated with egg. Standards, controls, and samples to be determined are pipetted in the "wells." Following an incubation and a wash phase, a polyclonal antibody marked with HRP (Horseradish-Peroxidase) is added. After a further incubation and wash phase, the substrate (Tetramethylbenzidin) for the enzyme reaction is added. The color reaction is halted through the addition of 0.5 M sulfuric acid (stop solution) and measured with a photometer with a wave length of 450 nm. The concentration of anti-egg is proportional to the intensity of the coloration. Using the standard curve, which is also measured, the concentration of the samples is calculated. |