Maier Analytik – Specific Antibodies in Stool

Designation	Medium	Method	Tests	Art. Nr.
Anti-Transglutaminase-ELISA	Stool	ELISA	96	01-10.095
One polyclonal antibody is used in the anti-transglutaminase-ELISA. A microtiter plate is coated with transglutaminase. Standards, controls, and samples to be determined are pipetted in the "wells." Following an incubation and a wash phase, a polyclonal antibody marked with HRP (Horseradish-Peroxidase) is added. After a further incubation and wash phase, the substrate (Tetramethylbenzidin) for the enzyme reaction is added. The color reaction is halted through the addition of 0.5 M sulfuric acid (stop solution) and measured with a photometer with a wave length of 450 nm. The concentration of anti-transglutaminase is proportional to the intensity of the coloration. Using the standard curve, which is also measured, the concentration of the samples is calculated. Sample volume: 100 µl Incubation: 1 + 1 + 0,5h h Packing insert: More information»
Anti-Transglutaminase (1-Punkt)	Stool	ELISA	96	01-12.095
One polyclonal antibody is used in the anti-gliadin ELISA. A microtiter plate is coated with gliadin. Calibrator, controls, and samples to be determined are pipetted in the "wells." Following an incubation and a wash phase, a polyclonal antibody marked with HRP (Horseradish-Peroxidase) is added. After a further incubation and wash phase, the substrate (Tetramethylbenzidin) for the enzyme reaction is added. The color reaction is halted through the addition of 0.5 M sulfuric acid (stop solution) and measured with a photometer with a wave length of 450 nm. The concentration of anti-gliadin is proportional to the intensity of the coloration. Using the calibrator, which is also measured, the concentration of the samples is calculated. Sample volume: 100 µl Incubation: 1 + 1 + 0,5 h Packing insert: More information»
Anti-Transglutaminase ILMA	Stool	ILMA	100	01-02.095
One polyclonal antibody that is highly specific against human immunglobuline is used in the anti-transglutaminase-ILMA. A polystyrol ball was coated with transglutaminase. Standards, controls, and samples to be determined are pipetted in the tubes together with a coated ball. Following incubation and wash phases, a polyclonal antibody marked with acridiniumester is added. Another incubation and wash step follows. Now the light emission is measured with a luminometer. The concentration of anti-transglutaminse is proportional to the height of light emission. Using the standard curve, which was also measured, the concentration of the samples is calculated. Sample volume: 100 µl Incubation: 2 + 2 h Packing insert: More information»
Anti-Gliadin ELISA	Stool	ELISA	96	01-10.092
One polyclonal antibody is used in the anti-gliadin-ELISA. A microtiter plate is coated with gliadin. Standards, controls, and samples to be determined are pipetted in the "wells." Following an incubation and a wash phase, a polyclonal antibody marked with HRP (Horseradish-Peroxidase) is added. After a further incubation and wash phase, the substrate (Tetramethylbenzidin) for the enzyme reaction is added. The color reaction is halted through the addition of 0.5 M sulfuric acid (stop solution) and measured with a photometer with a wave length of 450 nm. The concentration of anti-gliadin is proportional to the intensity of the coloration. Using the standard curve, which is also measured, the concentration of the samples is calculated. Sample volume: 100 µl Incubation: 2 + 2 h Packing insert: More information»
Anti-Gliadin (1-point-calibration)	Stool	ELISA	96	01-12.092
One polyclonal antibody is used in the anti-gliadin-ELISA. A microtiter plate is coated with gliadin. Calibrator, controls, and samples to be determined are pipetted in the "wells." Following an incubation and a wash phase, a polyclonal antibody marked with HRP (Horseradish-Peroxidase) is added. After a further incubation and wash phase, the substrate (Tetramethylbenzidin) for the enzyme reaction is added. The color reaction is halted through the addition of 0.5 M sulfuric acid (stop solution) and measured with a photometer with a wave length of 450 nm. The concentration of anti-gliadin is proportional to the intensity of the coloration. Using the calibrator, which is also measured, the concentration of the samples is calculated. Sample volume: 100 µl Incubation: 1 + 1 + 0,5 h Packing insert: More information»
Anti-Gliadin ILMA	Stool	ILMA	100	01-02.092
One polyclonal antibody that is highly specific against human immunglobuline is used in the Anti-Gliadin-ILMA. A polystyrol ball is coated with gliadin. Standards, controls, and samples to be determined are pipetted in the tubes together with a coated ball. Following incubation and wash phases, a polyclonal antibody marked with acridiniumester is added. Another incubation and wash step follows. Now the light emission is measured with a luminometer. The concentration of anti-gliadin is proportional to the height of light emission. Using the standard curve, which is also measured, the concentration of the samples is calculated. Sample volume: 100 µl Incubation: 2 + 2 h Packing insert: More information»
Anti-soy ELISA	Stool	ELISA	96	01-10.096
One polyclonal antibody is used in the anti-soy ELISA. A microtiter plate is coated with soy. Standards, controls, and samples to be determined are pipetted in the "wells." Following an incubation and a wash phase, a polyclonal antibody marked with HRP (Horseradish-Peroxidase) is added. After a further incubation and wash phase, the substrate (Tetramethylbenzidin) for the enzyme reaction is added. The color reaction is halted through the addition of 0.5 M sulfuric acid (stop solution) and measured with a photometer with a wave length of 450 nm. The concentration of anti-soya is proportional to the intensity of the coloration. Using the standard curve, which is also measured, the concentration of the samples is calculated. Sample volume: 100 µl Incubation: 1 + 1 + 0,5 h Packing insert: More information»
Anti-milk ELISA	Stool	ELISA	96	01-10.097
One polyclonal antibody is used in the anti-milk ELISA. A microtiter plate is coated with milk. Standards, controls, and samples to be determined are pipetted in the "wells." Following an incubation and a wash phase, a polyclonal antibody marked with HRP (Horseradish-Peroxidase) is added. After a further incubation and wash phase, the substrate (Tetramethylbenzidin) for the enzyme reaction is added. The color reaction is halted through the addition of 0.5 M sulfuric acid (stop solution) and measured with a photometer with a wave length of 450 nm. The concentration of anti-milk is proportional to the intensity of the coloration. Using the standard curve, which is also measured, the concentration of the samples is calculated. Sample volume: 100 µl Incubation: 1 + 1 + 0,5 h Packing insert: More information»
Anti-egg ELISA	Stool	ELISA	96	01-10.098
One polyclonal antibody is used in the anti-egg ELISA. A microtiter plate is coated with egg. Standards, controls, and samples to be determined are pipetted in the "wells." Following an incubation and a wash phase, a polyclonal antibody marked with HRP (Horseradish-Peroxidase) is added. After a further incubation and wash phase, the substrate (Tetramethylbenzidin) for the enzyme reaction is added. The color reaction is halted through the addition of 0.5 M sulfuric acid (stop solution) and measured with a photometer with a wave length of 450 nm. The concentration of anti-egg is proportional to the intensity of the coloration. Using the standard curve, which is also measured, the concentration of the samples is calculated. Sample volume: 100 µl Incubation: 1 + 1 + 0,5 h Packing insert: More information»

Specific Antibodies in Stool

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