» Assays
The intestinal mucosa is the largest surface area of our bodies. It is protected by specific and unspecific defense mechanisms. The specific immune system is essentially determined by the gut associated lymphoid tissue (GALT). All classes of immunoglobulins are secreted through this system. Evidence of the immune status can be made by determining immunoglobulins A, E, G, M, and secretory IgA.
| Designation | Medium | Method | Tests | Art. Nr. |
|---|---|---|---|---|
| Immunoglobulin A ELISA | Stool | ELISA | 96 | 01-10.040 |
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Two polyclonal antibodies are used in the immunoglobulin A ELISA that are highly specific against human intestinal immunoglobulin A. A microtiter plate is coated with a polyclonal rabbit antibody geared against immunoglobulin A. Standards, controls, and samples to be determined are pipetted in the "wells." Following an incubation and a wash phase, a polyclonal antibody marked with HRP (Horseradish-Peroxidase) is added. After a further incubation and wash phase, the substrate (Tetramethylbenzidin) for the enzyme reaction is added. The color reaction is halted through the addition of 0.5 M sulfuric acid (stop solution) and measured with a photometer with a wave length of 450 nm. The concentration of immunoglobulin A is proportional to the intensity of the coloration. Using the standard curve, which is also measured, the concentration of the samples is calculated. |
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| Immunoglobulin A - LIA | Stool | LIA | 100 | 01.01.040 |
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One polyclonal antibody is used in the immunglobuline A LIA that is highly specific against human albumin. A polystyrol ball was coated with a polyclonal rabbit antibody geared against immunglobuline A . Standards, controls, and samples to be determined are pipetted in the tubes together with a ball. The antigene (IgA) labled with acridiniumester is added. An incubation and wash phase follows. Now the light emission is measured with a luminometer. The concentration of IgA is proportional to the height of light emission. Using the standard curve, which was also measured, the concentration of the samples is calculated. |
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| Immunoglobulin E - ELISA | Stool | ELISA | 96 | 01-10.080 |
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Two polyclonal antibodies are used in the immunglobuline E ELISA that are highly specific against human intestinal immunglobuline E. A microtiter plate is coated with a polyclonal rabbit antibody geared against immunoglobulin E. Standards, controls, and samples to be determined are pipetted in the "wells." Following an incubation and a wash phase, a polyclonal antibody marked with HRP (Horseradish-Peroxidase) is added. After a further incubation and wash phase, the substrate (Tetramethylbenzidin) for the enzyme reaction is added. The color reaction is halted through the addition of 0.5 M sulfuric acid (stop solution) and measured with a photometer with a wave length of 450nm. The concentration of immunoglobulin E is proportional to the intensity of the coloration. Using the standard curve, which is also measured, the concentration of the samples is calculated.
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| Immunoglobulin E (1-point-calibration) | Stool | ELISA | 96 | 01-12.080 |
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Two polyclonal antibodies are used in the immunoglobulin E ELISA that are highly specific against human intestinal immunoglobulin E. A microtiter plate is coated with a polyclonal rabbit antibody geared against immunoglobulin E. Calibrator, controls, and samples to be determined are pipetted in the "wells." Following an incubation and a wash phase, a polyclonal antibody marked with HRP (Horseradish-Peroxidase) is added. After a further incubation and wash phase, the substrate (Tetramethylbenzidin) for the enzyme reaction is added. The color reaction is halted through the addition of 0.5M sulfuric acid (stop solution) and measured with a photometer with a wave length of 450nm. The concentration of immunoglobulin E is proportional to the intensity of the coloration. Using the calibrator, which is also measured, the concentration of the samples is calculated. |
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| Immunoglobulin E - ILMA | Stool | ILMA | 100 | 01-02.080 |
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Two polyclonal antibodies that are highly specific against human calprotectin A are used in the Immunglobuline E ILMA. A polystyrol ball was coated with a polyclonal rabbit antibody geared against Immunglobuline E. Standards, controls, and samples to be determined are pipetted in the tubes together with a coated ball. Following incubation and wash phases, a polyclonal antibody marked with acridiniumester is added. Another incubation and wash step follows. Now the light emission is measured with a luminometer. The concentration of Immunglobuline E is proportional to the height of light emission. Using the standard curve, which was also measured, the concentration of the samples is calculated. |
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| Immunoglobulin G - ELISA | Stool | ELISA | 96 | 01-10.081 |
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Two polyclonal antibodies are used in the immunglobuline G ELISA that are highly specific against human intestinal immunglobuline G. A microtiter plate is coated with a polyclonal rabbit antibody geared against immunoglobulin G. Standards, controls, and samples to be determined are pipetted in the "wells." Following an incubation and a wash phase, a polyclonal antibody marked with HRP (Horseradish-Peroxidase) is added. After a further incubation and wash phase, the substrate (Tetramethylbenzidin) for the enzyme reaction is added. The color reaction is halted through the addition of 0.5 M sulfuric acid (stop solution) and measured with a photometer with a wave length of 450 nm. The concentration of immunoglobulin G is proportional to the intensity of the coloration. Using the standard curve, which is also measured, the concentration of the samples is calculated. |
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| Immunoglobulin M - ELISA | Stool | ELISA | 96 | 01-10.082 |
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Two polyclonal antibodies are used in the immunoglobulin M ELISA that are highly specific against human intestinal immunoglobulin M. A microtiter plate is coated with a polyclonal rabbit antibody geared against immunoglobulin M. Standards, controls, and samples to be determined are pipetted in the "wells." Following an incubation and a wash phase, a polyclonal antibody marked with HRP (Horseradish-Peroxidase) is added. After a further incubation and wash phase, the substrate (Tetramethylbenzidin) for the enzyme reaction is added. The color reaction is halted through the addition of 0.5 M sulfuric acid (stop solution) and measured with a photometer with a wave length of 450 nm. The concentration of immunoglobulin M is proportional to the intensity of the coloration. Using the standard curve, which is also measured, the concentration of the samples is calculated. |
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| Immunoglobulin M (1 point calibration) | Stool | ELISA | 96 | 01-12.082 |
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Two polyclonal antibodies are used in the immunoglobulin M ELISA that are highly specific against human intestinal immunoglobulin M. A microtiter plate is coated with a polyclonal rabbit antibody geared against immunoglobulin M. Calibrator, controls, and samples to be determined are pipetted in the "wells." Following an incubation and a wash phase, a polyclonal antibody marked with HRP (Horseradish-Peroxidase) is added. After a further incubation and wash phase, the substrate (Tetramethylbenzidin) for the enzyme reaction is added. The color reaction is halted through the addition of 0.5 M sulfuric acid (stop solution) and measured with a photometer with a wave length of 450 nm. The concentration of immunoglobulin M is proportional to the intensity of the coloration. Using the calibrator, which is also measured, the concentration of the samples is calculated. |
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| Secretory Immunoglobulin A 1 - ELISA | Stool | ELISA | 96 | 01-10.041 |
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Two polyclonal antibodies are used in the immunoglobulin A 1 ELISA that are highly specific against human intestinal immunglobuline A 1. A microtiter plate is coated with a polyclonal rabbit antibody geared against immunglobuline A 1. Standards, controls, and samples to be determined are pipetted in the "wells." Following an incubation and a wash phase, a polyclonal antibody marked with HRP (Horseradish-Peroxidase) is added. After a further incubation and wash phase, the substrate (Tetramethylbenzidin) for the enzyme reaction is added. The color reaction is halted through the addition of 0.5 M sulfuric acid (stop solution) and measured with a photometer with a wave length of 450 nm. The concentration of immunoglobulin A 1 is proportional to the intensity of the coloration. Using the standard curve, which is also measured, the concentration of the samples is calculated. |
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| Secretory Immunoglobulin A1 + A 2 - ELISA | Stool | ELISA | 96 | 01-10.042 |
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Two polyclonal antibodies are used in the immunoglobulins A 1 + A 2 ELISA that are highly specific against human intestinal secretory immunoglobulins A 1 + A 2. A microtiter plate is coated with a polyclonal rabbit antibody geared against immunoglobulin A. Standards, controls, and samples to be determined are pipetted in the "wells." Following an incubation and a wash phase, a polyclonal antibody marked with HRP (Horseradish-Peroxidase) is added. After a further incubation and wash phase, the substrate (Tetramethylbenzidin) for the enzyme reaction is added. The color reaction is halted through the addition of 0.5 M sulfuric acid (stop solution) and measured with a photometer with a wave length of 450 nm. The concentration of immunoglobulins A 1 + A 2 is proportional to the intensity of the coloration. Using the standard curve, which is also measured, the concentration of the samples is calculated. |
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| Secretory IgA , total, ELISA | Stool | ELISA | 96 | 01-10.043 |
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Two polyclonal antibodies are used in the immunoglobulin A ELISA. One is highly specific against human intestinal immunoglobulin A and the other against the secretory component. A microtiter plate is coated with a polyclonal rabbit antibody geared against immunoglobulin A. Standards, controls, and samples to be determined are pipetted in the "wells." Following an incubation and a wash phase, a polyclonal antibody marked with HRP (Horseradish-Peroxidase) is added. After a further incubation and wash phase, the substrate (Tetramethylbenzidin) for the enzyme reaction is added. The color reaction is halted through the addition of 0.5 M sulfuric acid (stop solution) and measured with a photometer with a wave length of 450 nm. The concentration of immunoglobulin A is proportional to the intensity of the coloration. Using the standard curve, which is also measured, the concentration of the samples is calculated. |
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| Secretory IgA , total (1-point-calibration) | Stool | ELISA | 96 | 01-12.043 |
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Two polyclonal antibodies are used in the immunoglobulin A ELISA. One is highly specific against human intestinal immunoglobulin A and the other against the secretory component. A microtiter plate is coated with a polyclonal rabbit antibody geared against immunoglobulin A. Calibrator, controls, and samples to be determined are pipetted in the "wells." Following an incubation and a wash phase, a polyclonal antibody marked with HRP (Horseradish-Peroxidase) is added. After a further incubation and wash phase, the substrate (Tetramethylbenzidin) for the enzyme reaction is added. The color reaction is halted through the addition of 0.5M sulfuric acid (stop solution) and measured with a photometer with a wave length of 450 nm. The concentration of immunoglobulin A is proportional to the intensity of the coloration. Using the calibrator, which is also measured, the concentration of the samples is calculated. |
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