» Assays
Crohn's disease and ulcerative colitis are the principle types of inflammatory bowel diseases. It is important to note that Crohn's disease does not only affect the small intestine and large intestine, it can also affect the mouth, esophagus, stomach, and the anus, whereas ulcerative colitis primariliy affects the colon and the rectum.
Various gastrointestinal diseases result in an increased mucosal enteral protein loss. The quantitative detection of enteral protein loss is fundamental to the diagnosis.
| Designation | Medium | Method | Tests | Art. Nr. |
|---|---|---|---|---|
| Albumin, ELISA | Stool | ELISA | 96 | 01-10.060 |
|
Two polyclonal antibodies are used in the albumin ELISA that are highly specific against human albumin. A microtiter plate is coated with a polyclonal rabbit antibody geared against albumin. Standards, controls, and samples to be determined are pipetted in the "wells." Following an incubation and a wash phase, a polyclonal antibody marked with HRP (Horseradish-Peroxidase) is added. After a further incubation and wash phase, the substrate (Tetramethylbenzidin) for the enzyme reaction is added. The color reaction is halted through the addition of 0.5 M sulfuric acid (stop solution) and measured with a photometer with a wave length of 450nm. The concentration of albumin is proportional to the intensity of the coloration. Using the standard curve, which is also measured, the concentration of the samples is calculated. |
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| Albumin (1-point-calibration) | Stool | ELISA | 96 | 01-12.060 |
|
Two polyclonal antibodies are used in the albumin ELISA that are highly specific against human albumin. A microtiter plate is coated with a polyclonal rabbit antibody geared against albumin. Calibrator, controls, and samples to be determined are pipetted in the "wells". Following an incubation and a wash phase, a polyclonal antibody marked with HRP (Horseradish-Peroxidase) is added. After a further incubation and wash phase, the substrate (Tetramethylbenzidin) for the enzyme reaction is added. The color reaction is halted through the addition of 0.5M sulfuric acid (stop solution) and measured with a photometer with a wave length of 450 nm. The concentration of albumin is proportional to the intensity of the coloration. Using the calibrator, which is also measured, the concentration of the samples is calculated. |
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| Albumin, LIA | Stool | LIA | 100 | 01-01.060 |
|
One polyclonal antibody is used in the albumin LIA that is highly specific against human albumin. A polystyrol ball is coated with a polyclonal rabbit antibody geared against albumin. Standards, controls, and samples to be determined are pipetted in the tubes together with a ball. The antigene (albumin) labled with acridiniumesther is added. Incubation and wash phases follow. Now the light emission is measured with a luminometer. The concentration of albumin is proportional to the extent of light emission. Using the standard curve, which is also measured, the concentration of the samples is calculated. |
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| Alpha-1-Antitrypsin | Stool | ELISA | 96 | 01-10.030 |
|
In a first incubation step, the alpha 1 Antitrypsin in the samples is bound to polyclonal rabbit antibodies which are immobilized to the surface of the microtiter wells.To remove all unbound substances, a wash step is carried out. In a second incubation step, a peroxidase – labeled anti alpha 1 – antitrypsin antibody is added. After another washing step to remove all unbound substances, the solid phase is incubated with substrate (TMB). An acid stop solution is then added to stop the reaction. The color will change and must be measured at 450 nm in a microplate reader.
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| Alpha-1-Antitrypsin (1-point-calibration) | Stool | ELISA | 96 | 01-12.030 |
|
In a first incubation step, the alpha 1 Antitrypsin in the samples is bound to polyclonal rabbit antibodies which are immobilized to the surface of the microtiter wells.To remove all unbound substances, a washing step is carried out. In a second incubation step, a peroxidase – labeled anti alpha 1 –antitrypsin antibody is added. After another washing step to remove all unbound substances, the solid phase is incubated with substrate (TMB). An acid stop solution is then added to stop the reaction. The color will change and must be measured at 450 nm in a microplate reader. The intensity of the color is directly proportional to the concentration of alpha 1-antitrypsin in the sample. |
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| Alpha-1-Antitrypsin LIA | Stool | LIA | 100 | 01-01.030 |
|
One polyclonal antibody is used in the alpha-1-antitrypsin LIA that is highly specific against human albumin. A polystyrol ball was coated with a polyclonal rabbit antibody geared against alpha-1-antitrypsin . Standards, controls, and samples to be determined are pipetted in the tubes together with a ball. The antigene (alpha-1-antitrypsin ) labled with acridiniumester is added. An incubation and wash phase follows. Now the light emission is measured with a luminometer. The concentration of alpha-1-antitrypsin is proportional to the height of light emission. Using the standard curve, which was also measured, the concentration of the samples is calculated.
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| Lysozyme ELISA | Stool | ELISA | 96 | 01-10.020 |
|
Two polyclonal antibodies are used in the lysozyme ELISA that are highly specific against human lysozyme. A microtiter plate is coated with a polyclonal rabbit antibody geared against lysozyme. Standards, controls, and samples to be determined are pipetted in the "wells." Following an incubation and a wash phase, a polyclonal antibody marked with HRP (Horseradish-Peroxidase) is added. After a further incubation and wash phase, the substrate (Tetramethylbenzidin) for the enzyme reaction is added. The color reaction is halted through the addition of 0.5M sulfuric acid (stop solution) and measured with a photometer with a wave length of 450nm. The concentration of lysozyme is proportional to the intensity of the coloration. Using the standard curve, which was also measured, the concentration of the samples is calculated.
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| Lysozyme 1-point-calibration | Stool | ELISA | 96 | 01-12.020 |
|
Two polyclonal antibodies are used in the lysozyme ELISA that are highly specific against human lysozyme. A microtiter plate is coated with a polyclonal rabbit antibody geared against lysozyme. Calibrator, controls, and samples to be determined are pipetted in the "wells." Following an incubation and a wash phase, a polyclonal antibody marked with HRP (Horseradish-Peroxidase) is added. After a further incubation and wash phase, the substrate (Tetramethylbenzidin) for the enzyme reaction is added. The color reaction is halted through the addition of 0.5M sulfuric acid (stop solution) and measured with a photometer with a wave length of 450nm. The concentration of lysozyme is proportional to the intensity of the coloration. Using the calibrator, which is also measured, the concentration of the samples is calculated.
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| Lysozyme LIA | Stool | LIA | 100 | 01-01.020 |
|
One polyclonal antibody is used in the lysozyme LIA that is highly specific against human albumin. A polystyrol ball was coated with a polyclonal rabbit antibody geared against lysozyme. Standards, controls, and samples to be determined are pipetted in the tubes together with a ball. The antigene (lysozyme) labled with acridiniumester is added. An incubation and wash phase follows. Now the light emission is measured with a luminometer. The concentration of lysozyme is proportional to the height of light emission. Using the standard curve, which was also measured, the concentration of the samples is calculated. |
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