» Assays
Because many diseases are associated with exocrine pancreas insufficiencies (e.g. cystic fibrosis, alcohol abuse, physical trauma, fibrosis, etc.), early detection makes sense. Pancreas enzymes are released in the digestive tract and survive the intestinal passage unharmed. The pancreas enzymes in stool thus reflect the exocrine pancreas function.
| Designation | Medium | Method | Tests | Art. Nr. |
|---|---|---|---|---|
| Trypsin ELISA | Stool | ELISA | 96 | 01-10.071 |
|
Two polyclonal antibodies are used in the trypsin ELISA that are highly specific against human trypsin. A microtiter plate is coated with a polyclonal rabbit antibody geared against trypsin. Standards, controls, and samples to be determined are pipetted in the "wells." Following an incubation and a wash phase, a polyclonal antibody marked with HRP (Horseradish-Peroxidase) is added. After a further incubation and wash phase, the substrate (Tetramethylbenzidin) for the enzyme reaction is added. The color reaction is halted through the addition of 0.5 M sulfuric acid (stop solution) and measured with a photometer with a wave length of 450 nm. The concentration of trypsin is proportional to the intensity of the coloration. Using the standard curve, which is also measured, the concentration of the samples is calculated.
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| Trypsin-1-point calibration | Stool | ELISA | 96 | 01-12.071 |
|
Two polyclonal antibodies are used in the trypsin ELISA that are highly specific against human trypsin. A microtiter plate is coated with a polyclonal rabbit antibody geared against trypsin. Calibrator, controls, and samples to be determined are pipetted in the "wells." Following an incubation and a wash phase, a polyclonal antibody marked with HRP (Horseradish-Peroxidase) is added. After a further incubation and wash phase, the substrate (Tetramethylbenzidin) for the enzyme reaction is added. The color reaction is halted through the addition of 0.5M sulfuric acid (stop solution) and measured with a photometer with a wave length of 450 nm. The concentration of trypsin is proportional to the intensity of the coloration. Using the calibrator, which is also measured, the concentration of the samples is calculated. |
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| Pancreatic Amylase | Stool | ILMA | 100 | 01-02.070 |
|
Two polyclonal antibodies that are highly specific against human pancreatic amylase are used in the pancreatic amylase ILMA. A polystyrol ball was coated with a polyclonal rabbit antibody geared against pancreatic amylase. Standards, controls, and samples to be determined are pipetted in the tubes together with a coated ball. Following incubation and wash phases, a polyclonal antibody marked with acridiniumester is added. Another incubation and wash step follows. Now the light emission is measured with a luminometer. The concentration of pancreatic amylase is proportional to the height of light emission. Using the standard curve, which was also measured, the concentration of the samples is calculated. |
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